CT Value (Cycle Threshold) in RT-PCR: Lower Value - Higher Viral Load | Different from CT Score

Опубликовано: 22 Октябрь 2024
на канале: Examrace (UPSC, NET, NCERT, ICSE ...)

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Short for cycle threshold, Ct is a value that emerges during RT-PCR tests, the gold standard for detection of the SARS-CoV-2 coronavirus. In an RT-PCR test, RNA is extracted from the swab collected from the patient. It is then converted into DNA, which is then amplified. Amplification refers to the process of creating multiple copies of the genetic material — in this case, DNA. This improves the ability of the test to detect the presence of virus. Amplification takes place through a series of cycles — one copy becomes two, two becomes four, and so on — and it is after multiple cycles that a detectable amount of virus is produced.

Chapters:
0:00 CT Value (Cycle Threshold) in RT-PCR
1:21 Range for CT Value
1:48 RT-PCR
2:32 Cycle Threshold
4:11 High and Low Viral Load
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