Next Generation Sequencing Techniques and their applications

Опубликовано: 25 Ноябрь 2024
на канале: Dr. Vipin's Classroom

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As Next Generation Sequencing becomes a first stop for most analysis downstream - DNASeq, RNASeq, ChIPSeq, ATACSeq, FARESeq .... etc, here is an elaborate take on the evolution of sequencing techniques - from the first to second to the third Generation, from intermediate read length (Sanger) to short reads - Illumina and Ion Torrent, to finally long read sequencing - Nanopore and SMRT, from sequencing the first human genome in about 10 years to sequencing it in less than a day, from sequencing it for 3 billion dollars to less than a 1000 dollars - Be my guest on this journey most remarkable -

This is a recorded webinar of my presentation on 'Next Generation Sequencing Techniques and their applications' at Hindu College, University of Delhi

#NGS #Genomics #Sequencing #Nanopore #Illumina #Ion_Torrent #SMRT #Short_reads #Long_reads_sequencing #Genome #Next_Generation_Sequencing #lets_grow_together #vipins_e_classroom

0:00 Introduction
1:34 Genome Sequencing steps
5:01 Depth / Coverage
7:28 Polymerase based sequencing
9:38 Automated Sanger Sequencing
12:18 The evolution of Sequencing technologies
14:04 Contemporary Sequencing technologies
14:41 COMMON FEATURES OF II GENERATION SEQUEN
16:27 Virtual Terminator sequencing-Illumina, Sole
21:42 lon torrent
27:27 SMRT - Single Molecule Real Time Sequencing
29:11 Nanopore - Helicase based sequencing
30:02 Biological Nanopore
30:17 Nanopore - principle
31:03 Oxford Nanopore
31:59 Nanopore - advantages
32:28 The Game changer - ultra long read
34:16 NGS Broad Applications
35:18 Key features of NGS
36:22 Exome Sequencing
37:52 NGS based diagnostics - Targeted gene sequencing
41:13 Metagenomics - 16S RNA typing
41:42 Microbiome project
42:43 Pharmacogenomics
42:59 Purine Analogs: A Case Study in Pharmacogenetics
44:41 TPMT Variants and effect on drug dosage
48:00 Genome Sequencing Milestones
48:58 Largest genome ever sequenced
50:04 Short read aligners
50:45 References
51:05 Desired skill set